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HighYield T7 Biotin16 RNA Labeling Kit

貨號	RNT-101-BIO16	售價（元）	4425
規(guī)格	30T x 20 μl	CAS號

產(chǎn)品簡介
相關(guān)產(chǎn)品

產(chǎn)品信息

品牌	貨號	名稱	規(guī)格	單價
Jena Bioscience	RNT-101-BIO16	HighYield T7 Biotin16 RNA Labeling Kit	30T x 20 μl	4425.00

產(chǎn)品簡介

HighYield T7 Biotin16 RNA標記試劑盒設(shè)計用于通過體外轉(zhuǎn)錄產(chǎn)生帶有16個原子連接子的隨機生物素修飾RNA探針。這種探針非常適合原位雜交和Northern Blot實驗。標記原則類似于生物素RNA標記混合物（羅氏）的基本標記原則。使用優(yōu)化的反應(yīng)緩沖液和T7 RNA標記聚合酶混合物，生物素-16-UTP作為天然對應(yīng)物UTP的替代物有效地并入RNA。35%的生物素-16-UTP替代通常會在反應(yīng)和標記效率之間實現(xiàn)最佳平衡。然而，生物素-16-UTP/UTP比值的個體優(yōu)化可以通過單核苷酸格式輕松實現(xiàn)。由此產(chǎn)生的帶有16個原子連接子的生物素修飾RNA探針隨后可以被HRP或AP修飾的鏈親和素檢測到。

試劑盒包含足夠的試劑，用于30個標記反應(yīng)，每個反應(yīng)20μl（35%生物素-16-UTP替代物，1 mM ATP，GTP，CTP，0.65 mM UTP，0.35 mM生物素-16-UTP）。

Content:
HighYield T7 RNA Labeling Polymerase Mix
2x 40 μl, incl. RNase inhibitor and 50 % glycerol (v/v)

HighYield T7 Reaction Buffer
1x 200 μl (10x), HEPES-based

ATP - Solution
1x 100 μl (100 mM)

GTP - Solution
1x 100 μl (100 mM)

CTP - Solution
1x 100 μl (100 mM)

UTP - Solution
1x 100 μl (100 mM)

Biotin-16-UTP
1x 30 μl (10 mM)

T7 G-initiating control template (1.4 kbp)
1x 10 μl (200 ng/μl), 1.4 kbp PCR fragment plus T7 class III phi6.5 promotor resulting in approx. 1400 nt RNA transcript

PCR-grade water
1x 1.2 ml

DTT
1x 100 μl (100 mM)

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HighYield T7 Biotin16 RNA Labeling Kit