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MemGlow? NR4A Membrane Polarity Probe ex 553 em 637 50-300 slides (MEMBRIGHT

貨號(hào) MG06 售價(jià)(元) 6130
規(guī)格 50-300 slides CAS號(hào)
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MemGlow NR4A is a solvatochromic photostable plasma membrane-targeting dye with on/off switching that has been optimized for Super Resolution Point Accumulation for Imaging in Nanoscale Topography (SR-PAINT). Upon binding with a plasma membrane in a predominant liquid ordered (LO) phase NR4A exhibits a 45-50 nm wavelength shift relative to liquid disordered (Ld) phase enabling investigators to examine the nanoscale distribution of local chemical polarity in plasma membranes.

NR4A Membrane Polarity Probe Product Functions (MG06):

The NR4A Membrane Polarity Probe is the first probe that enables nanoscale cartography of the lipid order exclusively at the surface of live cells.

The NR4A Membrane Polarity Probe reveals nanoscopic protrusions and invaginations of lower Lipid Order in Plasma Membranes

High Specificity helps unveil rich nanoscale structural features of the plasma membrane

Weak Binders allow for better tracking of turn on events thanks to continuous flow exchange between the probe and membrane

** 2 nmol of MemGlow will produce a 20μM stock solution when reconstituted with 100 μl of anhydrous DMSO

Figure 1. Diagram of NR4A’s lipid order-dependent emission. 

Figure 2. Absorbance and excitation spectra of MemGlow NR4A diluted in DMSO to 4.8 μM. Absorbance peak detected at 553 nm with peak emission at 637 nm. 

Figure 3. A) Super resolution imaging of live HeLa cells labeled with 10 nM NR4A (false colored red). B) Widefield fluorescent imaging of live HeLa S1 cells labeled with 10 nM NR4A. HeLa S1 cells were imaged with a TRITC filter set, a digital CCD camera, and 100x oil objective (false colored red).

 

 

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