日本无码人妻丰满熟妇5g影院,人妻无码一区二区,国产精品一区二区在线播放,精品国产乱码久久久久久蜜柚,亚洲AV无码成人精品

歡迎蒞臨南京沃博生物科技有限公司官方網(wǎng)站!

Microtubules (Taxol Stabilized and Lyophilized)

貨號 MT002-A 售價(元) 6406
規(guī)格 4 x 500 μg CAS號
  • 產(chǎn)品簡介
  • 相關(guān)產(chǎn)品

Product uses:

Substrate for activation of kinesin ATPases (see Fig. 1)

Determination of IC50s for kinesin inhibitors

Used in Cytoskeleton, Inc’s kinesin ELIPA and End point ATPase assays (Cat. # BK060 & BK053)

Substrate for discovery and characterization of microtubule binding proteins (MAPs) (see Fig. 2)

Ideal for HTS applications (inquire for bulk rates)

Material:
       Stabilized microtubules (MTs) are supplied as a lyophilized powder. Microtubules have been prepared from porcine brain tubulin protein that is greater than 99% pure (Cat. # T240). These stringently quality controlled MTs provide highly reliable and reproducible results in assays that require MT substrates. The average MT length in this product is 2 μm. Using these pre-formed microtubules in assays depending on microtubules gives very reproducible results. In a microtubule activated kinesin ATPase assay (see Fig. 1), the CV between samples (Vmax) was less than 5% and the CV of Vmax between assays using different batches of MT001 was less than 8%.

Purity:
       The tubulin protein used to make pre-formed microtubules is >99% pure. See Cat. # T240.

Biological Activity:
       MTs serve as a substrate for kinesin motor proteins. Kinesin motor proteins orchestrate a wide range of kinetic events within a cell. They have been shown to move cargoes such as chromosomes and vesicles along MT tracks. Kinesins operate by utilizing the energy of ATP to hydrolysis, an activity that is greatly enhanced in the presence of MTs. An MT activated Enzyme Linked Inorganic Phosphate ATPase (ELIPA?) assay (Cat. # BK060) can therefore be used as a test for the biological activity of MT001 (Fig. 1). The motor protein used in this assay is Kinesin Heavy Chain motor domain (KHC: Cat. # KR01). MT001 is also an excellent substrate for detecting microtubule binding proteins, see Fig. 2. 

       Figure 1. MT activated kinesin ATPase reaction. Reactions were performed using the kinesin ELIPA assay Biochem? kit (Cat. # BK060). Reactions contained either MTs or KHC (Cat. # KR01) alone ("Reactions minus MTs or KHC") or the combination of MTs and KHC ("MTs and KHC reactions") . Triplicate reactions were measured in a spectrophotometer at 360 nm. Readings were taken once every 30 s for a total reaction time of 20 min. 

       Figure 2. Microtubule (MT) binding protein spin-down assay using MT002. >80% of MT002 (arrow: Tubulin) is in pellet (P) after spin-down. MAPs (Cat. # MAPF) bind to MTs and end up in pellet while BSA does not and stays in supernatant (S).

 

国产怮女视频免费观看 | 性感美女裸污污污视频网站 | 四川少妇BBw搡BBBB槡BBBB 四川少妇bbw搡bbbb搡bbbb | 无码精品人妻日韩A片下载 免费看AAAAA级少婬片 | 日韩在线视频中文 | 丰满老寡妇高潮免费无码 | 久久精品人妻一区二区三区蜜桃 | 白丝美女扒开双腿高潮叫爽娇喘视频 | 亚洲无码中文字幕国产 | 国产无码在线看免费看 | 亚洲成人视频在线观看无码 | 欧美一区二区三区喷汁尤物 | 亚洲一区二区三区乱码在线观看 | 91n.传媒二区| 少妇一夜三次一区二区 | 亚洲高清无码在线视频 | 成人午夜电影在线播放 | 国产精品探花一区二区在线观看 | 国产精品人妻无码久久久豆腐 | 近親相姦中文字幕A片 | 久一久二久三久在线观看视频 | 人妖欧美一区二区三区 | 国产精品 视频 | 免费看黄色视频麻豆网站 | 苍井优成人AV在线 | 中国老妇女爽到高潮 | 色秘 乱码一区二区三在线看 | 狠狠色综合7777之夜色撩人 | 精品国产精品无码A片久久妖精 | 黄色成人视频免费观看 | 白丝女疯狂 喷水自慰爽 | 很黄很污网站在线观看 | 日本一级婬片A片AAA免费 | 中文字幕制服丝袜一区二区三区 | 中文字幕亚州无码强奸乱伦亚州有码 | 午夜av日韩日逼网 | 国产91精品秘 入福利姬 | 国产高清 精品丝瓜 | 无码精品人妻一区二区三区影院 | 国产亚洲欧美一区二区 | 黄色三级视频在线观看 |